Gataca iLAS TIRF
Purpose:
- TIRF/ HiLo/ WF illumination
- Single-molecule imaging / tracking
- Super-resolution localization microscopy
- FRAP
Illumination / Imaging configuration: TIRF, HiLo / Widefield
Imaging contrasts: Fluorescence, phase contrast and transmitted light
Microscope stand: Inverted Nikon Ti2-E, motorized
Fluorescence excitation source: CW lasers (GATACA: https://www.gataca-systems.com/products/gataca-products/ilaunch/). 642nm@110mW; 568nm@140mW; 490nm@150mW; 410nm@100mW
Detection:
- Fluorescence: 1 or 2 channels (Optosplit with ByPass mode)
- Brightfield / phase contrast: 1 channel
Detection device: ORCA-Flash 4.0
| Camera | Andor iXon Ultra 897 | Retiga R1 |
| Technology | EM-CCD | CCD |
| Detection | Fluorescence | Phase contrast, Brightfield |
| Chip size | 512x512 pixels | 1360x1024 pixels |
| Pixel size | 16x16 µm | 6.45x6.45 µm |
| Dynamic range | 16-bit | 14-bit |
| Max frame rate* | 50 frames.s-1 | 25 frames.s-1 |
| Quantum efficiency | 95% @ 550 nm | 75% @ 600 nm |
*full chip, no binning
Acquisition software: Metamorph
Additional features:
- XY motorized stage
- Hardware Z-drift compensation (Nikon PFS)
- Incubation box for temperature control
- External phase contrast
- Stroboscopic illumination for single-molecule tracking
Objectives:
| Objective type | Mag/NA | Medium | Contrast | WB (mm) | Z-Drift compensation system | Pixel size (nm) |
planApoϒ | 20x / 0.75 | Air | DIC N2 | 1 | YES | 400 |
planFlour | 40x / 0.75 | Air | DIC N2 | 0.66 | / | 660 |
Apo TIRF | 100x / 1.49 | Oil | Fluo | 0.12 | YES | 125 |
Fluorescence filters single-channel detection:
Excitation dichroic filter | Emission filter Reference | FPbase spectra viewer |
Di03-R405/488/561/635-t1 | 525/50 FF03-525/50 | |
Di03-R561 | 568 LP BLP01-568R | |
Di03-R405/488/561/635-t1 | 609/54 FF01-609/54 | |
Di03-R405/488/561/635-t1 | 708/75 FF01-708/75 |
Fluorescence filters split-channel detection:
Emission filter Reference | FPbase spectra viewer |
FF01-525/50 | |
FF01-630/92 | |
FF01-600/52 | |
ET705/72 |
